Cytokine profiles in healthy animals and in cows with purulent-necrotic limb processes

One of the typical and mandatory reactions body to trauma or infectious agents for damage any tissues organs is acute phase reaction, which an induced increase in synthesis followed by blood a number proteins with immunological, bactericidal, antioxidant inhibitory functions. The purpose study determine levels cytokines clinically healthy cows, pigs dogs, as well cows purulent-necrotic processes limbs. pro-inflammatory anti-inflammatory IL-10 were determined serum dogs. Cows lesions limbs (n=26) divided into: 1st group (n=8) – form distal limbs; 2nd generalized lesions; 3 (n=10) recurrent foci area fingers. content TNF-α, IL-1ß interleukins was immunoenzymatic method according standard protocol. It established that TNF-α are significantly lower than IL-10, ratio IL-10:TNF-α 3.3:1, IL- 10: 1ß - 9.5:1, therefore, cattle under physiological norms, inherent cytokine profile. In pigs, profile turned out be most pronounced, indices them higher: 19.4:1; IL-10:IL-1β 13.9:1. between IL-10:IL-1ß extremely low 1.5:1, : 0.2:1, even lower, 8.8:1, therefore much totality indices. necrobacteriosis, compared animals, level 5.6 times higher (Р<0.001), 3.4 due their index 1.7 times, up 4.9:1. Under such conditions, increases only 1.8 (р<0.05). Animals characterized critical 16.8 17.8 (p <0.001), while remains unchanged form. Cytokine acquires value 0.4:1, 1:1. animals lesions, IL-1ß, especially characteristic. However, concentration quite high exceeds normal 12.6 (p<0.001). Therefore, various clinical forms necrobacterial have compensatory non-compensatory nature cytokinemia, imbalance functionality acute-phase insufficient capacity potential sick cows. Key words: cytokines, serum, inflammation.